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The influence of folate supplementation on global gene expression in normal colonic mucosa of subjects with colorectal adenoma
Author(s) -
Pufulete Maria,
Abbadi Reyad AlGhnaniem,
Arno Matthew,
Ewins Mark,
Green Caroline,
Astarloa Estibaliz AldecoaOtalora,
Sanders Tom,
Emery Peter
Publication year - 2013
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201200617
Subject(s) - colorectal adenoma , microarray , gene expression , adenoma , microarray analysis techniques , downregulation and upregulation , gene , colorectal cancer , biology , medicine , gastroenterology , pathology , cancer , genetics
Scope We determined whether short‐term supplementation with a physiological dose of folate alters global gene expression in the normal colonic mucosa of subjects with colorectal adenoma. Methods and results Fourteen subjects with adenoma, randomised to receive folic acid (400μg/day, n = 6) or placebo ( n = 8) for 10 weeks, had blood samples and colonic tissue biopsies collected before and after the intervention. RNA extracted from colonic tissue samples was used to determine global gene expression in the colon using A ffymetrix® M icroarray G ene C hips and real‐time RT ‐ PCR . Following intervention, 67 genes were upregulated and 13 genes were downregulated in the folic acid group, while 21 genes were upregulated and none were downregulated in the placebo group ( p < 0.05, adjusted for multiple testing). Thirty‐six genes were upregulated and 18 genes were downregulated in the folic acid group when compared with placebo, but none of these were statistically significant after adjustment for multiple testing. These genes are involved in multiple pathways, including cell cycle, signal transduction, cell differentiation, transport, cell division, cell motility, protein transport, and immune response. Conclusion These results indicate that while folic acid can modify gene expression, it is difficult to separate its effects from the natural variability in gene expression in the colon.