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Auraptene suppresses inflammatory responses in activated RAW 264 macrophages by inhibiting p38 mitogen‐activated protein kinase activation
Author(s) -
Lin Shan,
Hirai Shizuka,
Goto Tsuyoshi,
Sakamoto Tomoya,
Takahashi Nobuyuki,
Yano Masamichi,
Sasaki Takao,
Yu Rina,
Kawada Teruo
Publication year - 2013
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201200611
Subject(s) - proinflammatory cytokine , inflammation , adipose tissue , adipose tissue macrophages , tumor necrosis factor alpha , protein kinase a , chemistry , p38 mitogen activated protein kinases , insulin resistance , kinase , microbiology and biotechnology , pharmacology , endocrinology , biology , insulin , biochemistry , immunology , white adipose tissue
Scope Inflammation plays a key role in obesity‐related pathologies such as insulin resistance and type 2 diabetes. Hypertrophied adipocytes trigger the enhancement of macrophage infiltration and the release of various proinflammatory factors in obese adipose tissue. In this study, we examined whether auraptene, a citrus‐fruit–derived compound, could suppress the production of inflammatory factors that mediate the interaction between adipocytes and macrophages. Methods and results Experiments using a co‐culture system of 3T3‐L1 adipocytes and RAW264 macrophages showed that auraptene reduced the production of nitric oxide and tumor necrosis factor‐α. In RAW264 macrophages, auraptene also suppressed the inflammation induced by either LPS or the conditioned medium derived from 3 T 3‐ L 1 adipocytes. In addition, auraptene inhibited the phosphorylation of the p38 mitogen‐activated protein kinase and suppressed the production of proinflammatory mediators in activated macrophages. Conclusion Our findings indicate that auraptene exhibits anti‐inflammatory properties by suppressing the production of inflammatory factors that mediate the interaction between adipocytes and macrophages, suggesting that auraptene is a valuable food‐derived compound with a potential to attenuate chronic inflammation in adipose tissue and to improve obesity‐related insulin resistance.