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Effective suppression of azoxymethane‐induced aberrant crypt foci formation in mice with citrus peel flavonoids
Author(s) -
Lai ChingShu,
Li Shiming,
Liu Cheng Bin,
Miyauchi Yutaka,
Suzawa Michiko,
Ho ChiTang,
Pan MinHsiung
Publication year - 2013
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201200606
Subject(s) - azoxymethane , aberrant crypt foci , chemistry , carcinogenesis , nitric oxide synthase , nitric oxide , ornithine decarboxylase , cyclooxygenase , biochemistry , in vivo , microbiology and biotechnology , pharmacology , biology , colorectal cancer , cancer , enzyme , gene , colonic disease , genetics , organic chemistry
Citrus peel or its extract has been reported to exhibit a broad spectrum of biological activity. Herein, we report the first investigation of inhibitory effects of a formulated product from citrus peel extract, gold lotion ( GL ), on azoxymethane‐induced colonic tumorigenesis. We have demonstrated that oral feeding of GL decreased the number of aberrant crypt foci ( ACF ), particularly large size of ACF in colonic tissues of mice. Both gene and protein expression of inducible nitric oxide synthase (i NOS ) and cyclooxygenase‐2 ( COX ‐2) were suppressed by GL treatment. The in vivo data have revealed for the first time that the citrus peel extract– GL –is an effective antitumor agent mechanistically downregulating the protein levels of i NOS , COX ‐2, ornithine decarboxylase, vascular endothelial growth factor, and matrix metallopeptidase 9 in colonic tissues of mice, suggesting that GL is a novel functional natural product capable of preventing inflammation‐associated colon tumorigenesis.