Premium
Delayed bacterial colonization of the gut alters the host immune response to oral sensitization against cow's milk proteins
Author(s) -
Morin Stéphanie,
Fischer Romy,
PrzybylskiNicaise Laetitia,
Bernard Hervé,
Corthier Gérard,
Rabot Sylvie,
Wal JeanMichel,
Hazebrouck Stéphane
Publication year - 2012
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201200412
Subject(s) - immune system , sensitization , food allergy , mesenteric lymph nodes , biology , splenocyte , immunology , immunity , cholera toxin , allergy , microbiology and biotechnology
Scope Cow's milk allergy is the most prevalent food allergy in infants whose immune system development is critically stimulated during postnatal gut colonization by commensal bacteria. Allergenic potential of cow's milk β‐lactoglobulin ( BLG ) and caseins ( CAS ) was investigated in germ‐free ( GF ) BALB /c mice and in GF mice conventionalized ( CV d) at 6 weeks of age. Methods and results Oral sensitization to cow's milk in the presence of cholera toxin led to higher BLG ‐specific I g E , I g G 1, and I g G 2a responses in GF mice than in conventional ( CV ) mice. No significant difference was observed for CAS ‐specific I g E responses although I g G 1 responses to α S 1‐ and κ‐caseins were higher in GF mice than in CV mice. CV d mice, orally inoculated with fecal preparations from CV mice, also displayed biased antibody responses compared to CV mice. Secretion of T h2 cytokines by BLG ‐ and CAS ‐reactivated splenocytes of CV d mice was similar to that of GF mice whereas cytokine production by reactivated cells from mesenteric lymph nodes of CV d mice was equivalent to that of CV mice. Conclusion Oral sensitization to BLG and CAS was differentially affected by the absence of gut microbiota and delayed bacterial colonization altered persistently the host immune response to oral sensitization against food antigens.