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Epitope mapping of the major allergen from Atlantic cod in Spanish population reveals different IgE‐binding patterns
Author(s) -
PerezGordo Marina,
PastorVargas Carlos,
Lin Jing,
Bardina Ludmilla,
Cases Barbara,
Ibáñez Maria Dolores,
Vivanco Fernando,
CuestaHerranz Javier,
Sampson Hugh A.
Publication year - 2013
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201200332
Subject(s) - epitope , allergen , immunoassay , antigen , immunoglobulin e , biology , parvalbumin , gadus , immunology , allergy , antibody , fish <actinopterygii> , genetics , fishery
Scope IgE‐epitope mapping of allergens reveal important information about antigen components involved in allergic reactions. The peptide‐based microarray immunoassay has been used to map epitopes of some food allergens. We developed a peptide microarray immunoassay to map allergenic epitopes in parvalbumin from Atlantic cod (Gad m 1 ) , the most consumed cod species in Spain. Methods and results Sera from 13 fish‐allergic patients with specific IgE to cod parvalbumin were used. A library of overlapping peptides was synthesized, representing the primary sequence of Gad m 1. Peptides were used to analyze allergen‐specific IgE antibodies in patient sera. 100% of the patients recognized one antigenic region of 15 amino acids in length in Gad m 1. This region only partially correlated with one of the three antigenic determinants of Gad c 1 (Allergen M), parvalbumin from Baltic cod ( Gadus callarias ). In the 3D model of the protein, this region was located on the surface of the protein. Conclusion We have identified a relevant antigenic region in Gad m 1. This epitope could be considered as a severity marker and provides additional information to improve fish allergy diagnosis and the design of safe immunotherapeutic tools.