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Curcuminoids distinctly exhibit antioxidant activities and regulate expression of scavenger receptors and heme oxygenase‐1
Author(s) -
Kou MeiChun,
Chiou ShuYuan,
Weng ChingYi,
Wang Lisu,
Ho ChiTang,
Wu MingJiuan
Publication year - 2013
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201200227
Subject(s) - cd36 , heme oxygenase , gclm , chemistry , scavenger receptor , biochemistry , antioxidant , curcumin , hmox1 , receptor , heme , glutathione , enzyme , gclc , lipoprotein , cholesterol
Scope Curcumin (CUR), demethoxycurcumin (DMC), and bisdemethoxycurcumin (BDMC) have been demonstrated as having antioxidant, anticarcinogenic, and hypocholesterolemic activities. We report the diverse antiatherogenic effects and mechanisms of curcuminoids. Methods and results We found that CUR was the most potent antioxidant against copper‐mediated LDL oxidation as measured by thiobarbituric acid‐reactive substances assay, oxidized LDL (oxLDL) ELISA, and electrophoretic mobility. CUR upregulated heme oxygenase‐1, modifier subunit of glutamate‐cysteine ligase (GCLM), and CD36 expression in undifferentiated THP‐1 cells, supporting the possible involvement of Nrf2 pathway in CD36 expression. Monocyte‐to‐macrophage differentiation plays a vital role in early atherogenesis. BDMC reduced oxLDL uptake most effectively, while CUR was the best inhibitor for CD36, scavenger receptor A, and lectin‐like oxidized LDL receptor‐1 expression during phorbol 12‐myristate 13‐acetate (PMA)‐induced THP‐1 differentiation. In PMA‐differentiated THP‐1 macrophages, CUR and DMC effectively induced heme oxygenase‐1 expression, but attenuated oxLDL‐induced CD36 expression, leading to decreased oxLDL uptake. Conclusion This result indicates curcuminoids, despite structural similarities, exert different atheroprotective effects. Curcuminoids, especially CUR and DMC, are hormetic compounds, which induce Phase II enzyme expression and confer resistance to PMA‐ and oxLDL‐induced scavenger receptor expression and activity.