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Dose‐dependent absorption of chlorogenic acids in the small intestine assessed by coffee consumption in ileostomists
Author(s) -
Erk Thomas,
Williamson Gary,
Renouf Mathieu,
Marmet Cynthia,
Steiling Heike,
Dionisi Fabiola,
Barron Denis,
Melcher Ralf,
Richling Elke
Publication year - 2012
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201200222
Subject(s) - chemistry , urine , crossover study , absorption (acoustics) , excretion , metabolism , food science , polyphenol , chlorogenic acid , glucuronidation , small intestine , medicine , trigonelline , endocrinology , zoology , pharmacology , biochemistry , enzyme , biology , antioxidant , physics , alternative medicine , microsome , pathology , acoustics , placebo
Scope Until now, the question of how the ingested doses of chlorogenic acids ( CGA ) from coffee influence their absorption and metabolism remains unresolved. To assess absorption in the small intestine, we performed a dose‐response study with a randomized, double‐blinded, crossover design with ileostomist subjects. Methods and results After a polyphenol‐free diet, the volunteers consumed, on three separate occasions, coffee with different total CGA contents (high 4525 μmol; medium 2219 μmol; low 1053 μmol). CGA concentrations in plasma, ileal effluent, and urine were subsequently determined by HPLC ‐ DAD ‐ ESI ‐ MS and ‐ ESI ‐ MS / MS . The results show that the consumption of higher CGA concentrations leads to a faster ileal excretion. This corresponds to a renal excretion of 8.0 ± 4.9% (high), 12.1 ± 6.7% (medium), and 14.6 ± 6.8% (low) of total CGA and metabolites. Glucuronidation of CGA became slightly greater with increasing dose. After enzyme treatment, the area under the curve (AUC) 0–8h for CGA metabolites in plasma was 4412 ± 751 nM × h 0–8 −1 (high), 2394 ± 637 nM × h 0–8 −1 (medium), 1782 ± 731 nM × h 0–8 −1 (low), respectively. Additionally, we were able to identify new metabolites of CGA in urine and ileal fluid. Conclusion We conclude that the consumption of high CGA concentrations via coffee might influence the gastrointestinal transit time and consequently affect CGA absorption and metabolism.