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The kinetics of urinary fumonisin B 1 excretion in humans consuming maize‐based diets
Author(s) -
Riley Ronald T.,
Torres Olga,
Showker Jency L.,
Zitomer Nicholas C.,
Matute Jorge,
Voss Kenneth A.,
Gelineauvan Waes Janee,
Maddox Joyce R.,
Gregory Simon G.,
AshleyKoch Allison E.
Publication year - 2012
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201200166
Subject(s) - urine , excretion , mycotoxin , fumonisin b1 , fumonisin , zoology , chemistry , population , food science , urinary system , medicine , biology , biochemistry , environmental health
Scope Fumonisins ( FB ) are mycotoxins found in maize. The purpose of this study was to (i) determine the relationship between FB 1 , FB 2 , and FB 3 intake and urinary excretion in humans, (ii) validate a method to isolate urinary FB on C 18 ‐ SPE cartridges for international shipment, and (iii) test the method using samples from G uatemala. Methods and results Volunteers ( n = 10) consumed 206 grams/day of tortillas and biscuits prepared from masa flour and a product containing maize flour. Volunteers estimated their daily urine output and samples were analyzed for FB 1 , FB 2 , and FB 3 and hydrolyzed FB 1 . Only FB 1 was detected in urine suggesting lower absorption of FB 2 and FB 3 . Excretion was highly variable peaking soon after consumption began and decreasing rapidly after consumption stopped. Within 5 days after consumption ended, FB 1 was not detected in urine. In a study with eight volunteers, the average total urinary FB 1 was 0.5% of the intake. FB 1 was detected in 61% (107/177) of the samples collected in G uatemala. Conclusion The results support the use of urinary FB 1 to assess ongoing exposure in popula‐tion‐based studies. However, relating the FB 1 concentration in urine to dietary intake of FB by individual subjects will be complicated due to interindividual variability and the rapidity of clearance.