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Delipidating effect of resveratrol metabolites in 3 T 3‐ L 1 adipocytes
Author(s) -
Lasa Arrate,
Churruca Itziar,
Eseberri Itziar,
AndrésLacueva Cristina,
Portillo María P.
Publication year - 2012
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201100772
Subject(s) - resveratrol , chemistry , sirtuin 1 , glucuronide , metabolite , biochemistry , downregulation and upregulation , gene
Scope Due to the low bioavailability of resveratrol, determining whether its metabolites exert any beneficial effect is an interesting issue. Methods and results 3 T 3‐ L 1 maturing pre‐adipocytes were treated from day 0 to day 8 of differentiation and mature adipocytes for 24 h on day 12 with 1, 10, or 25 μM of resveratrol or its metabolites. Triacylglycerols were assessed by spectrophotometry and gene expression by real time RT‐PCR. Resveratrol, trans ‐resveratrol‐4′‐ O ‐glucuronide and trans ‐resveratrol‐3‐ O ‐sulfate reduced triacylglycerol content in maturing pre‐adipocytes at 25 μM. In mature adipocytes, both resveratrol and its glucuronide metabolites, though not sulfate metabolite, reduced triacylglycerol content, although resveratrol was more effective than them. Resveratrol and the three metabolites reduced C / EBP β m RNA levels. Trans ‐resveratrol‐3‐ O ‐sulfate also reduced C / EBP ‐α, peroxisome proliferator‐activated receptor γ ( PPAR ‐γ), and lipoprotein lipase ( LPL) expression. In mature adipocytes, resveratrol increased ATGL , CPT ‐1, deacetylase sirtuin 1 ( SIRT ‐1), and PGC 1‐α expression. Trans ‐resveratrol‐3‐ O ‐glucuronide reduced m RNA levels of FASN and increased those of SIRT ‐1. Trans ‐resveratrol‐4′‐ O ‐glucuronide increased HSL and SIRT ‐1 m RNA levels. Trans ‐resveratrol‐3‐ O ‐sulfate did not change gene expression. Conclusion The present study shows for the first time the delipidating effect of (i) resveratrol metabolites in maturing pre‐adipocytes and (ii) glucuronide metabolites in mature adipocytes. This suggests that both resveratrol and resveratrol metabolites may be involved in the anti‐obesity effect of this polyphenol.