Premium
Regulation of colon gene expression by vitamin B 6 supplementation
Author(s) -
Toya Keigo,
Hirata Atsuko,
Ohata Tomomi,
Sanada Yohei,
Kato Norihisa,
Yanaka Noriyuki
Publication year - 2012
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201100707
Subject(s) - gene , gene expression , vitamin , biology , food science , endocrinology , genetics
Scope Previous studies have shown that vitamin B 6 supplementation suppresses the development of colonic aberrant crypt foci ( ACF ), precursor lesions of colon cancer, and cell proliferation in mice receiving the colonic carcinogen, azoxymethane ( AOM ). This study investigated the molecular mechanism of these effects of dietary vitamin B 6. Methods and results To date, the mechanism by which ACF s develop is not yet fully understood. In a search for factors that play a critical role during ACF development, we examined colon gene expression during early stage of ACF development in AOM ‐treated mice using DNA microarray analysis. AOM treatment significantly upregulated m RNA closely related to mast cell and cytotoxic T ‐cell activity. This study also investigated the effect of vitamin B 6 supplementation on colon gene expression in AOM ‐treated mice. We found that vitamin B 6 supplementation downregulates C d8a and C cl8 m RNA expression, suggesting these candidate genes may play a protective role against colonic ACF development. Furthermore, we examined genomic affects of dietary vitamin B 6, and showed that R eg3γ m RNA expression in colons is downregulated by vitamin B 6. Conclusion This study provides an insight into the genomic activities of dietary vitamin B6 that may be protective against colon tumor development.