Premium
Lycopene attenuates LPS ‐induced TNF‐α secretion in macrophages and inflammatory markers in adipocytes exposed to macrophage‐conditioned media
Author(s) -
Marcotorchino Julie,
Romier Beatrice,
Gouranton Erwan,
Riollet Celine,
Gleize Beatrice,
MalezetDesmoulins Christiane,
Landrier JeanFrançois
Publication year - 2012
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201100623
Subject(s) - adipose tissue , macrophage , tumor necrosis factor alpha , lipopolysaccharide , lycopene , adipose tissue macrophages , proinflammatory cytokine , chemokine , chemistry , adipocyte , macrophage activating factor , inflammation , cytokine , macrophage inflammatory protein , microbiology and biotechnology , biology , endocrinology , immunology , biochemistry , white adipose tissue , in vitro , antioxidant
Scope Adipose tissue is infiltrated by an increasing number of macrophages during the development of obesity. These immune cells are suspected to be a major source of TNF ‐α that interferes with adipocyte function. Because lycopene possesses anti‐inflammatory properties, we hypothesize that lycopene could reduce the production of TNF ‐α by macrophages and thus interfere in the cross‐talk between macrophages and adipocytes. Methods and results We demonstrated that physiological concentrations of lycopene were able to attenuate the lipopolysaccharide ( LPS )‐mediated induction of TNF ‐α in RAW 264.7 macrophages, at both the m RNA and protein levels. The molecular mechanism was studied. It appeared that the LPS ‐activation of both JNK and NF ‐κ B signaling pathways was modulated by lycopene. The anti‐inflammatory effects of lycopene on macrophages were accompanied by a decrease in LPS ‐stimulated macrophage migration in the presence of lycopene. Furthermore, lycopene decreased macrophage conditioned medium‐induced proinflammatory cytokine, acute phase protein, and chemokine m RNA expression in 3 T 3‐ L 1 adipocytes. Conclusion These data indicate that lycopene displayed an anti‐inflammatory effect on macrophages that beneficially impacted adipocyte function. Thus, these results suggest that lycopene could block the vicious cycle that occurs between adipocytes and macrophages in adipose tissue during obesity.