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Molecular and immunological characterization of Mus a 5 allergen from banana fruit
Author(s) -
Aleksic Ivana,
Popovic Milica,
Dimitrijevic Rajna,
Andjelkovic Uros,
Vassilopoulou Emilia,
Sinaniotis Athanassios,
AtanaskovicMarkovic Marina,
Lindner Buko,
Petersen Arnd,
Papadopoulos Nikolaos G.,
GavrovicJankulovic Marija
Publication year - 2012
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201100541
Subject(s) - complementary dna , biology , allergen , peptide sequence , molecular cloning , molecular mass , amino acid , musa acuminata , microbiology and biotechnology , open reading frame , gene isoform , allergy , biochemistry , gene , genetics , immunology , enzyme
Scope Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of β‐1,3‐glucanase from banana ( Musa acuminata ), denoted as Mus a 5, were performed Methods and results The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI‐TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33451±67 Da. Two Mus a 5 isoforms (p I 7.7 and 8.0) were detected by 2D immunoblot with an identical N‐terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration‐dependent manner Conclusion Mus a 5 is a functional allergen and a candidate for the component‐resolved allergy diagnosis of banana allergy.