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Multi‐parametric approach to identify coffee components that regulate mechanisms of gastric acid secretion
Author(s) -
Rubach Malte,
Lang Roman,
Seebach Elisabeth,
Somoza Mark M.,
Hofmann Thomas,
Somoza Veronika
Publication year - 2012
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201100453
Subject(s) - gastric acid , secretion , chemistry , h(+) k(+) exchanging atpase , caffeine , receptor , biochemistry , biology , endocrinology , atpase , enzyme
Scope Chlorogenic acid (CA), caffeine (CAFF), pyrogallol (PYR), catechol (CAT), β N ‐alkanoyl‐hydroxytryptamides (C5HT) and N ‐methylpyridinium (N‐MP) were evaluated for their influence on mechanisms of gastric acid secretion as single compounds and in biomimetic mixtures. Methods and results Compounds were tested in coffee representative concentrations. Human gastric cancer cells (HGT‐1) were used to study the proton secretory activity by Ussing chamber experiments and FACS analysis. For activation of EGFr, Akt1, ERK1/2, ATF‐2 and cAMP levels, we performed pathway screening assays. Time‐dependent expression of related genes were determined by real‐time PCR. Part of the data was used for neural network modeling to identify the most relevant compounds. N‐MP increased the expression of the anti‐secretory somatostatin receptor by 114%, whereas C5HT decreased its expression by 52%. N‐MP down‐regulated the pro‐secretory CHRM3 receptor by 36% and the H + ,K + ‐ATPase by 36%. CAFF stimulated the secretory activity in the functional assays, whereas N‐MP and CA decreased proton secretion. After applying a pathway analysis, we were able to discriminate between CAFF, CA, CAT, C5HT, PYR and histamine‐activating EGFr signaling and N‐MP‐associated ERK1/2 signaling. Conclusion By applying a multi‐parametric approach, N‐MP was shown to effectively down‐regulate mechanisms of gastric acid secretion in human parietal gastric cells.