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Metabolites and tissue distribution of resveratrol in the pig
Author(s) -
AzorínOrtuño María,
YáñezGascón María J.,
Vallejo Fernando,
Pallarés Francisco J.,
Larrosa Mar,
Lucas Ricardo,
Morales Juan C.,
TomásBarberán Francisco A.,
GarcíaConesa María T.,
Espín Juan C.
Publication year - 2011
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201100140
Subject(s) - resveratrol , metabolite , ex vivo , glucuronide , cecum , metabolism , chemistry , glucuronidation , lymph , urine , gastrointestinal tract , biology , medicine , biochemistry , microsome , pathology , enzyme , in vitro
Scope : trans ‐Resveratrol (RES) and/(or) its metabolites exert many effects in vivo. Our aim was to study the metabolism and tissue distribution of RES using the pig, a mammal physiologically close to humans. Methods and results : Forty‐seven tissues, organs and fluids were analyzed 6 h after intragastric RES administration (5.9 mg/kg body weight) using HPLC‐MS/MS. Twelve RES and seven dihydroresveratrol (DH‐RES) metabolites were detected. DH‐RES was the main metabolite in cecum, colon and rectum, whereas RES‐3‐ O ‐glucuronide was the most abundant one in fluids and organs. Approximately 74.5% of the total RES administered was recovered in the form of RES, DH‐RES and derived metabolites (65.1% along the gastrointestinal tract, 7.7% in urine, 1.2% in bile and 0.5% in organs). We report here, for the first time, the occurrence of RES ribosyl‐sulfate derivative, DH‐RES diglucuronide, DH‐RES sulfoglucuronide and DH‐RES disulfate as well as the metabolic profile of RES and DH‐RES in the aorta, lymph, lymph node, ovaries, uterus, cerebellum, pancreas, urinary bladder tissue, fat and muscle. Conclusion : This study contributes to the clarification of the metabolism and tissue distribution of RES and could help to further understand the mechanisms underlying its effects.