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The inhibitory effects of 5‐hydroxy‐3,6,7,8,3′,4′‐hexamethoxyflavone on human colon cancer cells
Author(s) -
Qiu Peiju,
Guan Huashi,
Dong Ping,
Guo Shanshan,
Zheng Jinkai,
Li Shiming,
Chen Yun,
Ho ChiTang,
Pan MinHsiung,
McClements David Julian,
Xiao Hang
Publication year - 2011
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201100070
Subject(s) - umbilical vein , colorectal cancer , cancer cell , matrigel , angiogenesis , cancer , cancer research , western blot , microbiology and biotechnology , chemistry , biology , in vitro , biochemistry , medicine , gene
Scope: Previously, we reported that 5‐hydroxy‐3,6,7,8,3′,4′‐hexamethoxyflavone (5HHMF), a polymethoxyflavone found in citrus peels, potently inhibited the growth of multiple human colon cancer cells. Herein, we further investigated the anti‐cancer mechanisms of 5HHMF in human colon cancer cells. Methods and results: Colony formation assay revealed that 5HHMF dose dependently inhibited colony formation of multiple colon cancer cells. Western blot analysis demonstrated 5HHMF decreased nuclear β‐catenin levels and increased the E‐cadherin level in a dose‐dependent manner. 5HHMF also modified plasma membrane‐associated proteins, such as K‐Ras, EGFR, and their downstream effectors, such as Akt. Moreover, treatments with 5HHMF inhibited nuclear translocation of NF‐κB, which may contribute to its anti‐cancer effects. Add‐back study showed that the inhibitory effect of 5HHMF was not associated with the production of reactive oxygen species (ROS). In addition, 5HHMF treatment inhibited the capillary tube formation of human umbilical vein endothelial cells (HUVECs) on matrigel, suggesting a potential anti‐tumor angiogenesis effect. Conclusion: Our results demonstrated that 5HHMF suppressed multiple oncogenic molecular events in colon cancer cells.