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Effect of supplementation with vitamin D 3 on glucose production pathways in human subjects
Author(s) -
O'Sullivan Aifric,
Balducci Daniele,
Paradisi Francesca,
Cashman Kevin D.,
Gibney Michael J.,
Brennan Lorraine
Publication year - 2011
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201000653
Subject(s) - glycogenolysis , medicine , endocrinology , glycogen , carbohydrate metabolism , gluconeogenesis , insulin , vitamin , metabolism , chemistry , urine
Scope: Research reports suggest that vitamin D affects glucose and insulin metabolism; however, the exact mechanisms are unclear. 2 H NMR analysis of monoacetone glucose (MAG) after tracer administration provides a non‐invasive method of profiling hepatic glucose metabolism. This study examined the effects of supplementation with vitamin D 3 on contribution of glycogenolysis to glucose production. Methods and results: Tracer administration and biofluid collections were performed with eight healthy females before and following a 4‐wk vitamin D 3 administration period. Following an overnight fast subjects ingested deuterated water and acetaminophen. Full void urine samples were collected after 4 h. 2 H NMR spectra of urinary monoacetone glucose were acquired to determine the contribution of glycogenolysis to glucose production. The mean contribution of glycogenolysis to glucose production was 60±13%. Supplementation with vitamin D 3 had no effect on hepatic glucose production. Regression analysis revealed a significant relationship between carbohydrate intake and the contribution of glycogenolysis (β=0.914, p =0.004). Conclusion: In conclusion, we saw no changes in the percentage contribution of glycogenolysis following supplementation with vitamin D 3 . The reproducibility of our results and the non‐invasive nature of the method highlight the potential for this method in assessing mechanistic modes of action in future nutritional interventions.