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Apigetrin induces erythroid differentiation of human leukemia cells K562: Proteomics approach
Author(s) -
Tsolmon Soninkhishig,
Nakazaki Eri,
Han Junkyu,
Isoda Hiroko
Publication year - 2011
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201000650
Subject(s) - k562 cells , biology , cellular differentiation , differentiation therapy , downregulation and upregulation , cell cycle , leukemia , microbiology and biotechnology , cancer cell , proteomics , cancer research , cell , fetal hemoglobin , cell cycle checkpoint , cell culture , cancer , immunology , biochemistry , fetus , gene , genetics , pregnancy , acute promyelocytic leukemia , retinoic acid
Scope: Induction of cancer‐cell differentiation is an alternative approach for cancer chemotherapy. There are numerous studies that diets containing an abundance of fruits and vegetables have protection against cancers, and the main agents thought to provide such protective effect are flavonoids. In this study we used apigetrin as a possible cell differentiation inducer and chronic leukemia cells K562 for their pluripotent differentiating potency. Methods and results: Prolonged treatment with 75 μM apigetrin induced erythroid differentiation of K562 cells with specific marker glycophorin A expression and fetal hemoglobin synthesis in treated cells, which was accompanied with G 2 /M arrest. Proteomics data revealed the downregulation of several proteins expression involved in cell cycle regulation, protein synthesis and nuclear import and export of signaling molecules. Conclusion: This is the first evidence that natural compound apigetrin may induce cancer cell differentiation thus could be one of the possible explanations of its antitumor effects.