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Stabilization of small heterodimer partner mRNA by grape seed procyanidins extract in cultured hepatocytes
Author(s) -
Inoue Jun,
Tanaka Minako,
Nanmoku Manami,
Yashiro Takuya,
Sato Ryuichiro
Publication year - 2011
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201000489
Subject(s) - messenger rna , chemistry , grape seed , microbiology and biotechnology , postprandial , biochemistry , small heterodimer partner , grape seed extract , biology , gene , transcription factor , food science , endocrinology , medicine , alternative medicine , pathology , nuclear receptor , insulin
Scope: Consumption of dietary grape seed procyanidins extract (GSPE) has improved the plasma lipid profile in humans and experimental animals. The effect of GSPE on the reduction of the postprandial plasma triglyceride (TG) levels has been attributed to the activation of the small heterodimer partner (SHP). GSPE increases SHP gene expression in rat liver and the TG‐lowering effect of GSPE is abolished in SHP‐deficient mice. However, the mechanism by which GSPE increases SHP mRNA levels remains unclear. This study addressed the effect of GSPE on SHP mRNA stability. Methods and results: The present study shows for the first time that SHP mRNA is rapidly degraded, as measured by actinomycin D‐based mRNA chase experiments, and GSPE transiently stabilizes SHP mRNA in HepG2 cells. This degradative effect was completely abolished with 2 h of prolonged treatment with GSPE. However, treatment of fresh HepG2 cells with a pretreated GSPE‐containing medium also stabilized SHP mRNA, indicating that GSPE inactivation is not responsible for the transient effects that GSPE has on SHP mRNA stability. Conclusion: SHP expression is intricately controlled by mRNA stabilization, which is transiently increased by GSPE, along with at the transcriptional and posttranslational levels.