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9‐oxo‐10(E),12(E)‐octadecadienoic acid derived from tomato is a potent PPAR α agonist to decrease triglyceride accumulation in mouse primary hepatocytes
Author(s) -
Kim YoungIl,
Hirai Shizuka,
Takahashi Haruya,
Goto Tsuyoshi,
Ohyane Chie,
Tsugane Taneaki,
Konishi Chiaki,
Fujii Takashi,
Inai Shuji,
Iijima Yoko,
Aoki Koh,
Shibata Daisuke,
Takahashi Nobuyuki,
Kawada Teruo
Publication year - 2011
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201000264
Subject(s) - triglyceride , agonist , chemistry , peroxisome proliferator activated receptor , primary (astronomy) , endocrinology , medicine , pharmacology , biochemistry , receptor , biology , cholesterol , physics , astronomy
Scope: Tomato is one of the most common crops worldwide and contains many beneficial compounds that improve abnormalities of lipid metabolism. However, the molecular mechanism underlying the effect of tomato on lipid metabolism is unclear. It has been commonly accepted that peroxisome proliferator‐activated receptor α (PPARα) is one of the most important targets for ameliorating abnormalities of lipid metabolism. Therefore, we focused on the activation of PPARα and attempted to detect active compounds activating PPARα in tomato. Methods and results: To identify such active compounds, we screened fractions of tomato extracts using PPARα luciferase reporter assay. One fraction, rechromatographed‐fraction eluted in 57 min (RF57), significantly increased PPARα reporter activity, in which a single compound is detected by LC/MS analysis. On the basis of LC/MS and NMR analyses, we determined the chemical structure of the active compound in RF57 as 9‐oxo‐10(E),12(E)‐octadecadienoic acid (9‐oxo‐ODA). The RF57 fraction significantly increased the mRNA expression levels of PPARα target genes involved in fatty acid oxidation and O 2 consumption in mouse primary hepatocytes. Furthermore, RF57 inhibited cellular triglyceride accumulation in the hepatocytes. Conclusion: These findings suggest that tomatoes containing 9‐oxo‐ODA that acts on PPARα are valuable for ameliorating abnormalities of lipid metabolism.