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Colonic availability of polyphenols and D ‐(−)‐quinic acid after apple smoothie consumption
Author(s) -
Hagl Stephanie,
Deusser Hannah,
Soyalan Buelent,
Janzowski Christine,
Will Frank,
Dietrich Helmut,
Albert Franz Werner,
Rohner Simone,
Richling Elke
Publication year - 2011
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.201000252
Subject(s) - quinic acid , polyphenol , food science , chemistry , ellagic acid , ileostomy , chlorogenic acid , starter , biochemistry , antioxidant
Scope : The aim of this study was to determine the amounts of polyphenols and D ‐(−)‐quinic acid reaching the ileostomy bags of probands (and thus the colon in healthy humans) after ingestion of apple smoothie, a beverage containing 60% cloudy apple juice and 40% apple puree. Methods and results : Ten healthy ileostomy subjects each ingested 0.7 L of apple smoothie (a bottle). Their ileostomy bags were collected directly before and 1, 2, 4, 6 and 8 h after smoothie consumption, and the polyphenol and D ‐(−)‐quinic acid contents of the ileostomy fluids were examined using HPLC‐DAD and HPLC‐MS/MS. The total polyphenol and D ‐(−)‐quinic acid content of the apple smoothie was determined to be 1955.6±124.6 mg/0.7 L, which is very high compared to cloudy apple juices. The most abundant substances found in the ileostomy bags were oligomeric procyanidins (705.6±197.9 mg), D ‐(−)‐quinic acid (363.4±235.5 mg) and 5‐caffeoylquinic acid (76.7±26.8 mg). Overall recovery of ingested polyphenols and D ‐(−)‐quinic acid in the ileostomy bags was 63.3±16.1%. Conclusions: The amounts of polyphenol and D ‐(−)‐quinic acids reaching the ileostomy bags are considerably higher after apple smoothie consumption than after the consumption of cloudy apple juice or cider. These results suggest that the food matrix might affect the colonic availability of polyphenols, and apple smoothies could be more effective in the prevention of chronic colon diseases than both cloudy apple juice and apple cider.