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Food processing increases casein resistance to simulated infant digestion
Author(s) -
Dupont Didier,
Mandalari Giuseppina,
Mollé Daniel,
Jardin Julien,
RoletRépécaud Odile,
Duboz Gabriel,
Léonil Joëlle,
Mills Clare E. N.,
Mackie Alan R.
Publication year - 2010
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200900582
Subject(s) - digestion (alchemy) , casein , food science , glycosylation , chemistry , biochemistry , biology , chromatography
The objective of this study was to determine whether processing could modify the resistance of casein (CN) to digestion in infants. A range of different dairy matrices was manufactured from raw milk in a pilot plant and subjected to in vitro digestion using an infant gut model. Digestion products were identified using MS and immunochemical techniques. Results obtained showed that CNs were able to resist digestion, particularly κ‐ and αs 2 ‐CN. Resistant areas were identified and corresponded to fragments hydrophobic at pH 3.0 (gastric conditions) and/or carrying post‐translational modifications (phosphorylation and glycosylation). Milk processing led to differences in peptide patterns and heat treatment of milk tended to increase the number of peptides found in digested samples. This highlights the likely impact of milk processing on the allergenic potential of CNs.