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Disposition of hop prenylflavonoids in human breast tissue
Author(s) -
Bolca Selin,
Li Jinghu,
Nikolic Dejan,
Roche Nathalie,
Blondeel Phillip,
Possemiers Sam,
De Keukeleire Denis,
Bracke Marc,
Heyerick Arne,
van Breemen Richard B.,
Depypere Herman
Publication year - 2010
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200900519
Subject(s) - xanthohumol , glucuronidation , adipose tissue , chemistry , medicine , breast tissue , endocrinology , hop (telecommunications) , pharmacokinetics , metabolism , breast cancer , biochemistry , enzyme , microsome , cancer , computer network , computer science
Hop‐derived products may contain xanthohumol (XN), isoxanthohumol (IX), and the potent phytoestrogen 8‐prenylnaringenin (8‐PN). To evaluate the potential health effects of these prenylflavonoids on breast tissue, their concentration, nature of metabolites, and biodistribution were assessed and compared with 17β‐estradiol (E 2 ) exposure. In this dietary intervention study, women were randomly allocated to hop ( n =11; 2.04 mg XN, 1.20 mg IX, and 0.1 mg 8‐PN per supplement) or control ( n =10). After a run‐in of ≥4 days, three supplements were taken daily for 5 days preceding an aesthetic breast reduction. Blood and breast biopsies were analyzed using HPLC‐ESI‐MS/MS. Upon hop administration, XN and IX concentrations ranged between 0.72 and 17.65 nmol/L and 3.30 and 31.50 nmol/L, and between 0.26 and 5.14 pmol/g and 1.16 and 83.67 pmol/g in hydrolyzed serum and breast tissue, respectively. 8‐PN however, was only detected in samples of moderate and strong 8‐PN producers (0.43–7.06 nmol/L and 0.78–4.83 pmol/g). Phase I metabolism appeared to be minor (∼10%), whereas extensive glucuronidation was observed (> 90%). Total prenylflavonoids showed a breast adipose/glandular tissue distribution of 38/62 and their derived E 2 ‐equivalents were negligible compared with E 2 in adipose (384.6±118.8 fmol/g, p =0.009) and glandular (241.6±93.1 fmol/g, p <0.001) tissue, respectively. Consequently, low doses of prenylflavonoids are unlikely to elicit estrogenic responses in breast tissue.