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The regulation of jejunal induction of the maltase–glucoamylase gene by a high‐starch/low‐fat diet in mice
Author(s) -
Mochizuki Kazuki,
Honma Kazue,
Shimada Masaya,
Goda Toshinao
Publication year - 2010
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200900467
Subject(s) - maltase , cdx2 , enhancer , homeobox , endocrinology , acetylation , gene , medicine , histone , messenger rna , biology , chemistry , gene expression , microbiology and biotechnology , biochemistry , enzyme
Abstract Maltase and glucoamylase are derived from the same mRNA and are responsible for digestion of starch in the small intestine. Their jejunal activities in rodents are induced by a high‐starch/low‐fat (HS)‐diet. However, it is unknown whether jejunal expression of the maltase–glucoamylase ( Mgam ) gene is enhanced by the HS‐diet. In this study, we found that jejunal Mgam mRNA was increased by a HS‐diet in mice. We showed that the HS‐diet increased acetylation of histones, bindings of a coactivator, Creb binding protein (CREBBP), and the transcriptional factors caudal type homeobox 2 (CDX2) and HNF1 homeobox (HNF1) in the promoter/enhancer and transcriptional regions of Mgam gene. This suggests that the increase in the jejunal activity of maltase and glucoamylase caused by a HS‐diet in mice is regulated at the mRNA level through histone acetylation and binding of CREBBP, CDX2 and HNF1 in the promoter/enhancer and transcriptional regions of Mgam gene.