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Acute and chronic effects of dietary fatty acids on cholecystokinin expression, storage and secretion in enteroendocrine STC‐1 cells
Author(s) -
Hand Katharine V.,
Bruen Christine M.,
O'Halloran Fiona,
Giblin Linda,
Green Brian D.
Publication year - 2010
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200900343
Subject(s) - cholecystokinin , conjugated linoleic acid , linoleic acid , secretion , enteroendocrine cell , arachidonic acid , endocrinology , medicine , fatty acid , eicosapentaenoic acid , metabolism , biology , intracellular , biochemistry , chemistry , polyunsaturated fatty acid , hormone , endocrine system , enzyme , receptor
Cholecystokinin (CCK) is a peptide hormone secreted from the I‐cells of the intestine and it has important physiological actions related to appetite regulation and satiety. In this study we used STC‐1 cells to investigate the effects of common dietary‐derived fatty acids (FAs) on I‐cell secretory function and metabolism. We extend earlier studies by measuring the acute and chronic effects of 11 FAs on CCK secretion, cellular CCK content, CCK mRNA levels, cellular DNA synthesis, cellular viability and cytotoxicity. FAs were selected in order to assess the importance of chain length, degree of saturation, and double bond position and conformation. The results demonstrate that secretory responses elicited by dietary FAs are highly selective. For example, altering the conformation of a double bond from cis to trans ( i.e. oleic acid versus elaidic acid) completely abolishes CCK secretion. Lauric acid appears to adversely affect I‐cell metabolism and arachidonic acid suppresses DNA synthesis. Our studies reveal for the first time that conjugated linoleic acid isoforms are particularly potent CCK secretagogues, which also boost intracellular stores of CCK. These actions of conjugated linoleic acid may explain satiating actions observed in dietary intervention studies.