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3,3′‐Dihydroxyisorenieratene prevents UV‐induced formation of reactive oxygen species and the release of protein‐bound zinc ions in human skin fibroblasts
Author(s) -
Lutter Kaya,
De Spirt Silke,
Kock Sebastian,
Kröncke KlausDietrich,
Martin HansDieter,
Wagener Tanja,
Stahl Wilhelm
Publication year - 2010
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200900044
Subject(s) - intracellular , reactive oxygen species , chemistry , zinc , antioxidant , oxidative stress , biochemistry , biophysics , biology , organic chemistry
3,3′‐Dihydroxyisorenieratene (DHIR) is a structurally unusual carotenoid exhibiting bifunctional antioxidant properties. It is synthesized by Brevibacterium linens , used in dairy industry for the production of red smear cheeses. The compound protects cellular structures against photo‐oxidative damage and inhibits the UV‐dependent formation of thymidine dimers. Here we show that DHIR prevents a UV‐induced intracellular release of zinc ions from proteins in human dermal fibroblasts. The effect is correlated with a decreased formation of intracellular reactive oxygen species. In contrast, zinc release from cellular proteins induced by hyperthermia is not affected by pretreatment of cells with the antioxidant DHIR. It is suggested that the intracellular zinc release upon UV irradiation is due to oxidative modifications of the zinc ligands in proteins ( e.g. cysteine) and that protection by DHIR is due to intracellular scavenging of reactive oxygen species generated in photo‐oxidation.