Premium
Protein hydrolysates from β‐conglycinin enriched soybean genotypes inhibit lipid accumulation and inflammation in vitro
Author(s) -
MartinezVillaluenga Cristina,
Dia Vermont P.,
Berhow Mark,
Bringe Neal A.,
Gonzalez de Mejia Elvira
Publication year - 2009
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200800473
Subject(s) - chemistry , nitric oxide synthase , nitric oxide , lipase , foam cell , inflammation , lipid droplet , biochemistry , macrophage , in vitro , enzyme , biology , immunology , organic chemistry
Obesity is a worldwide health concern and a well recognized predictor of premature mortality associated with a state of chronic inflammation. The objective was to evaluate the effect of soy protein hydrolysates (SPH) produced from different soybean genotypes by alcalase (SAH) or simulated gastrointestinal digestion (SGIH) on lipid accumulation in 3T3‐L1 adipocytes. The anti‐inflammatory effect of SPH produced by alcalase on LPS‐induced macrophage RAW 264.7 cell line was also investigated. SAH (100 μM) derived from soybean enriched in β‐conglycinin (BC) (up to 47% total protein) decreased lipid accumulation (33–37% inhibition) through downregulation of gene expression of lipoprotein lipase (LPL) and fatty acid synthase (FAS). SGIH (100 μM) inhibited lipid accumulation to a lesser extent (8–14% inhibition) through inhibition of LPL gene expression. SAH (5 μM) decreased the production of nitric oxide (NO) (18–35%) and prostaglandin E 2 (PGE 2 ) (47–71%) and the expression of inducible nitric oxide synthase (iNOS) (31–53%) and cycloxygenase‐2 (COX‐2) (30–52%). This is the first investigation showing that soy hydrolysates inhibit LPS‐induced iNOS/NO and COX‐2/PGE 2 pathways in macrophages. Soybeans enriched in BCs can provide hydrolysates that limit fat accumulation in fat cells and inflammatory pathways in vitro and therefore warrant further studies as a healthful food.