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Metabolic response of soy pinitol on lipid‐lowering, antioxidant and hepatoprotective action in hamsters fed‐high fat and high cholesterol diet
Author(s) -
Choi MyungSook,
Lee MiKyung,
Jung Un Ju,
Kim HyeJin,
Do GeoyngMin,
Park Yong Bok,
Jeon SeonMin
Publication year - 2009
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200800241
Subject(s) - medicine , endocrinology , cholesterol , adiponectin , antioxidant , paraoxonase , adipose tissue , chemistry , lipid metabolism , apolipoprotein b , biology , insulin resistance , insulin , oxidative stress , biochemistry
This study was performed to investigate the lipid‐lowering, antioxidant, and hepato‐protective effects of pinitol in dose‐dependent manners in hamsters fed‐high fat and high cholesterol (HFHC) diet. Pinitol supplementation (0.05%, P‐I and 0.1% pinitol, P‐II) with an HFHC diet (10% coconut oil plus 0.2% cholesterol) for 10 wks significantly lowered the white adipose tissue weights, hepatic lipid droplets, plasma glucose, total‐cholesterol, nonHDL‐cholesterol, total‐cholesterol/HDL‐cholesterol ratio, and hepatic lipid levels. Whereas it significantly increased the brown adipose tissue weight, plasma HDL‐cholesterol, apolipoprotein A‐I (apo A‐I) concentrations, paraoxonase (PON) activity, and/or mRNA expression, compared to the HFHC control group. Plasma insulin and adiponectin levels were significantly lower and higher, respectively, in both P‐I and P‐II groups than the HFHC control group. Dietary pinitol significantly inhibited hepatic HMG‐CoA reductase, acyl‐CoA:cholesterol acyltransferase (ACAT), and cytochrome P4502E1 (CYP2E1) activities without altering their mRNA expressions compared to the control group. Pinitol significantly elevated the hepatic antioxidant enzyme activities, whereas it also significantly reduced the hepatic lipid peroxide and H 2 O 2 production. Accordingly, these results indicate that both 0.05 and 0.1% pinitol supplementation may improve the lipid and antioxidant metabolism in HFHC diet‐fed hamsters. In particular, pinitol supplementation was very effective on the elevation of antiatherogenic factors, including plasma HDL‐cholesterol, apo A‐I, adiponectin, and PON.