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Characterization of Bet v 1‐related allergens from kiwifruit relevant for patients with combined kiwifruit and birch pollen allergy
Author(s) -
Oberhuber Christina,
Bulley Sean M.,
BallmerWeber Barbara K.,
Bublin Merima,
Gaier Sonja,
DeWitt Åsa Marknell,
Briza Peter,
Hofstetter Gerlinde,
Lidholm Jonas,
Vieths Stefan,
HoffmannSommergruber Karin
Publication year - 2008
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200800146
Subject(s) - actinidia deliciosa , actinidia , actinidia chinensis , pollen , allergy , allergen , botany , recombinant dna , biology , chemistry , horticulture , biochemistry , immunology , gene
Abstract Allergy to kiwifruit appears to have become more common in Europe and elsewhere during the past several years. Seven allergens have been identified from kiwifruit so far, with actinidin, kiwellin and the thaumatin‐like protein as the most relevant ones. In contrast to other fruits, no Bet v 1 homologues were characterized from kiwifruit so far. We cloned, purified, and characterized recombinant Bet v 1‐homologous allergens from green (Actinidia deliciosa, Act d 8) and gold ( Actinidia chinensis , Act c 8) kiwifruit, and confirmed the presence of its natural counterpart by inhibition assays. Well‐characterized recombinant Act d 8 and Act c 8 were recognized by birch pollen/kiwifruit (confirmed by double‐blind placebo‐controlled food challenge) allergic patients in IgE immunoblots and ELISA experiments. The present data point out that Bet v 1 homologues are allergens in kiwifruit and of relevance for patients sensitized to tree pollen and kiwifruit, and might have been neglected so far due to low abundance in the conventional extracts used for diagnosis.