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Selenoprotein deficiency enhances radiation‐induced micronuclei formation
Author(s) -
Baliga Manjeshwar S.,
DiwadkarNavsariwala Veda,
Koh Timothy,
Fayad Raja,
Fantuzzi Giamila,
Diamond Alan M.
Publication year - 2008
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200800020
Subject(s) - chemoprotective , selenoprotein , micronucleus test , dna damage , genome instability , lncap , selenoprotein p , micronucleus , glutathione peroxidase , biology , gpx4 , transfection , transgene , gpx1 , chemistry , microbiology and biotechnology , cancer research , cancer cell , cancer , oxidative stress , dna , biochemistry , genetics , cell culture , toxicity , gene , superoxide dismutase , organic chemistry
The availability of selenium and the levels of specific selenoproteins might affect cancer risk by influencing the ability of DNA damaging agents to cause genomic instability and mutations. Transgenic mice that express reduced levels of selenoproteins and previously shown to be more susceptible to pathology associated with cancer development were used to study this possibility. These mice were exposed to X‐rays and DNA damage assessed in the erythrocytes, where micronuclei formation was higher compared to the same cells obtained from irradiated wild‐type controls. To determine whether the selenoprotein glutathione peroxidase‐1 (GPx‐1) might be involved in this protection, its levels were reduced by siRNA targeting in LNCaP human prostate cells. UV‐induced micronuclei frequency was higher in these cells compared to control‐transfected cells. These results indicate a role for selenoproteins in protecting DNA from damage and support human data implicating GPx‐1 as a possible target of the chemoprotective effect of selenium.