Premium
Identification of four IgE‐reactive proteins in raspberry ( Rubus ideaeus L.)
Author(s) -
Marzban Gorji,
Herndl Anita,
Kolarich Daniel,
Maghuly Fatemeh,
Mansfeld Agata,
Hemmer Wolfgang,
Katinger Hermann,
Laimer Margit
Publication year - 2008
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200700518
Subject(s) - polyclonal antibodies , immunoglobulin e , blowing a raspberry , biology , epitope , rubus , biochemistry , allergen , chemistry , microbiology and biotechnology , antibody , botany , immunology , allergy , food science
IgE‐reactive proteins in raspberry ( Rubus ideaus L.) were identified using PCR, RT‐PCR, 2‐DE and MS/MS peptide sequencing. Specific polyclonal antibodies and patient sera were used in Western blotting to identify crossreactive epitopes. Initially, two potential allergens Rub i 1 and Rub i 3 were detected using PCR, showing high sequence identity to proteins in Rosaceous species like Mal d 1 and Mal d 3 from apple, Pru av 1 and Pru av 3 from cherry and Pru p 1 and Pru p 3 from peach. Furthermore, de novo identified peptides of a protein band at about 30 kDa reacting with most of the patient sera tested (> 80%) revealed a high sequence homology with class III chitinases. Raspberry chitinase, when subjected to glycoproteomic analysis, showed typical complex plant‐type N ‐glycans with a core α1,3 fucose and a β1,2 xylose at least at one position, indicating the presence of crossreacting carbohydrate determinants (CCDs). Finally, MS/MS analysis revealed an IgE‐reactive raspberry cyclophilin, homologous to Bet v 7. Results obtained suggest that the consumption of raspberries might be responsible for adverse reactions in sensitised individuals.