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Free Zn 2+ enhances inhibitory effects of EGCG on the growth of PC‐3 cells
Author(s) -
Sun Shili,
He Guoqing,
Yu Haining,
Yang Junguo,
Borthakur Devajit,
Zhang Lancui,
Shen Shengrong,
Das Undurti N
Publication year - 2008
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200700172
Subject(s) - chemistry , zinc , inhibitory postsynaptic potential , epigallocatechin gallate , antioxidant , cell growth , membrane , membrane permeability , cell , biochemistry , biophysics , polyphenol , biology , endocrinology , organic chemistry
Epigallocatechin‐3‐gallate (EGCG), a major component of green tea, has both preventive and therapeutic beneficial actions in prostate cancer. In the present study, we compared the growth inhibitory effects and the antioxidant and ability to modify cell membrane permeation of zinc‐EGCG complex and Zn 2+ /EGCG mixture on androgen‐insensitive prostate cancer (PC‐3) cells. It was noted that free Zn 2+ enhanced the growth inhibitory effects of EGCG on PC‐3 cells at 160 μmol/L concentration, whereas zinc‐EGCG complex was ineffective. EGCG showed potent free radical scavenging ability in the presence of Zn 2+ . EGCG in the presence of Zn 2+ was more effective than EGCG alone in enhancing the permeability of the cell membrane, whereas zinc‐EGCG complex had no effect on PC‐3 cell membrane permeability. These results indicate that though Zn 2+ enhanced the action of EGCG on PC‐3 cells, zinc‐EGCG complex is highly unlikely to be formed in the presence of Zn 2+ and EGCG to explain the potentiating action of Zn 2+ on the growth inhibitory property of EGCG on PC‐3 cells.