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The efficacy of protective effects of tannic acid, gallic acid, ellagic acid, and propyl gallate against hydrogen peroxide‐induced oxidative stress and DNA damages in IMR‐90 cells
Author(s) -
Chen ChingHsein,
Liu TsanZon,
Chen ChinHui,
Wong Chung Hang,
Chen ChiHung,
Lu FungJou,
Chen Ssu Ching
Publication year - 2007
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200600230
Subject(s) - oxidative stress , gallic acid , lipid peroxidation , ellagic acid , tannic acid , chemistry , propyl gallate , dna damage , reactive oxygen species , biochemistry , glutathione , hydrogen peroxide , antioxidant , catalase , intracellular , polyphenol , dna , enzyme , organic chemistry
There is increasing evidence that reactive oxygen species (ROS) are intimately involved in the oxidative damage of tissues for a wide variety of pulmonary diseases. Thus, it is desirable to search for chemopreventive agents that can counteract ROS‐mediated injury to the pulmonary tissues. Using a human lung fibroblast IMR‐90 cells as the experimental model, we first demonstrated that nearly 90% of intracellular ROS could be removed when H 2 O 2 ‐treated cells (200 μM) simultaneously incubated with 10 μg/mL of tannic acid (TA), gallic acid (GA), ellagic acid (EA), and propyl gallate (PA). Using C 11 ‐BODIPY 581/591 as a lipid peroxidation probe, we also attested that all these compounds examined (10 μg/mL) could alleviate H 2 O 2 ‐evoked lipid peroxidation phenomena. Next, we examined the protective effects of these compounds on the depletion of intracellular glutathione (iGSH) in H 2 O 2 ‐treated cells using CMF‐DA probe. Interestingly, PA was demonstrated to be the only compound that could effectively protect the integrity of iGSH from being depleted by this system. Finally, the protective effects of these compounds against oxidative DNA damage were evaluated using 8‐oxoguanine formation as a marker. Our data indicated that all four compounds suppressed the formation of 8‐oxoguanine effectively. Taken together, our data suggested that TA, GA, EA, and PA can protect cells from oxidative stress.