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Heme oxygenase induction by cyanidin‐3‐O‐β‐glucoside in cultured human endothelial cells
Author(s) -
Sorrenti Valeria,
Mazza Francesco,
Campisi Agata,
Di Giacomo Claudia,
Acquaviva Rosaria,
Vanella Luca,
Galvano Fabio
Publication year - 2007
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200600204
Subject(s) - heme oxygenase , enos , oxidative stress , nitric oxide , heme , nitric oxide synthase , chemistry , endothelial dysfunction , endothelial nos , biochemistry , medicine , endocrinology , biology , enzyme
The aim of the present research was to investigate the effect of cyanidin‐3‐O‐β‐glucoside (C3G) on heme oxygenase‐1 (HO‐1), endothelial nitric oxide synthase (eNOS), inducible NOS (iNOS) and dimethylarginine dimethylamino hydrolase‐2 (DDAH‐2) expression in cultured endothelial cells. Different concentrations (0.00625–250 μM) of C3G were tested in order to investigate possible beneficial and harmful effects of C3G. Our data demonstrated that C3G increased the induction of eNOS and HO‐1 in a dose‐dependent manner. Higher concentration (62.5–250 μM) also resulted in increase of isoprostane, cGMP and PGE 2 levels and in induction of iNOS with consequent oxidative stress. In conclusion, our data evidence that C3G may exert various protective effects against endothelial dysfunction, whereas potentially harmful effects of C3G appear to be limited to concentrations very difficult to be reached in physiological conditions unless there is abundant oral supplementation.