Premium
Urinary 2‐ethyl‐3‐oxohexanoic acid as major metabolite of orally administered 2‐ethylhexanoic acid in human
Author(s) -
Stingel Dana,
Feldmeier Peter,
Richling Elke,
Kempf Michael,
Elss Sandra,
Labib Samira,
Schreier Peter
Publication year - 2007
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200600136
Subject(s) - metabolite , catabolism , urine , chemistry , metabolism , decarboxylation , urinary system , pharmacology , biochemistry , medicine , endocrinology , catalysis
Human metabolism of 2‐ethylhexanoic acid (2‐EHA), which is a known metabolite of important phthalates, was investigated using 2‐EHA‐contaminated food. The results of our studies reveal that the major catabolic pathway of 2‐EHA in human is β‐oxidation. The dominant final urinary metabolite was identified and quantified as 3‐oxo‐2‐ethylhexanoic acid (3‐oxo‐2‐EHA), but only after immediate methylation of the extract from urine and prior to GC‐MS analysis. Former studies without the precaution of immediate methylation had found 4‐heptanone as the major metabolite, which is obviously an artifact arising from the decarboxylation of 3‐oxo‐2‐EHA.