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Functional analyses of neutrophil‐like differentiated cell lines under a hyperglycemic condition
Author(s) -
OyaIto Tomoko,
Naitou Hirotaka,
Masuda Shuichi,
Kinae Naohide,
Ohashi Norio
Publication year - 2008
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200600122
Subject(s) - stimulation , reactive oxygen species , phagocytosis , intracellular , vacuole , phorbol , cell culture , medicine , respiratory burst , endocrinology , incubation , chemistry , microbiology and biotechnology , biology , biochemistry , signal transduction , protein kinase c , cytoplasm , genetics
Diabetic patients are prone to severe bacterial infections. The functional alterations of neutrophils by hyperglycemia are thought to be partially responsible for such infections. In this study, we investigated the functional changes of neutrophil‐like differentiated cell lines (dHL‐60, dTHP‐1, and dNB‐4) by treatment with 5.5 mM, 11 mM, or 35 mM of glucose. In dHL‐60 cells, the incubation with high glucose (35 mM) resulted in the enhancement of cell aggregation, the suppression of cellular fragility, the induction of reactive‐oxygen species (ROS) production by phorbol myristate acetate (PMA) stimulation, and the impairment of phagocytosis. In dTHP‐1 cells, the treatment with higher glucose generated the suppression of cellular fragility and extremely impaired phagocytosis (by 35 mM), and induced ROS production due to PMA stimulation (by 11 mM). Furthermore, the higher glucose exposure to dNB‐4 cells enlarged intracellular vacuoles (by 35 mM) and induced ROS production due to PMA stimulation (by 11 mM). Since the ROS generation of those cells was enhanced only after PMA stimulation under the higher glucose conditions, glucose may have a priming effect rather than a triggering effect. These extraordinary sensitivities caused by the higher glucose treatments may reflect the dysfunction or overactivation of neutrophils.