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Characterization of filamentous fungi isolated from Moroccan olive and olive cake: Toxinogenic potential of Aspergillus strains
Author(s) -
Roussos Sevastianos,
Zaouia Nabila,
Salih Ghislane,
TantaouiElaraki Abdelrhafour,
Lamrani Khadija,
Cheheb Mostafa,
Hassouni Hicham,
Verhé Fréderic,
PerraudGaime Isabelle,
Augur Christopher,
IsmailiAlaoui Mustapha
Publication year - 2006
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200600005
Subject(s) - geotrichum , aspergillus niger , paecilomyces , biology , aspergillus flavus , penicillium , aspergillus fumigatus , aspergillus , acremonium , microbiology and biotechnology , ochratoxin a , food science , mucor , aspergillus ochraceus , ochratoxin , citrinin , mycotoxin , botany
During the 2003 and 2004 olive oil production campaigns in Morocco, 136 samples from spoiled olive and olive cake were analyzed and 285 strains were isolated in pure culture. Strains included 167 mesophilic strains belonging to ten genera: Penicillium, Aspergillus, Geotrichum, Mucor, Rhizopus, Trichoderma, Alternaria, Acremonium, Humicola, Ulocladium as well as 118 thermophilic strains isolated in 2003 and 2004, mainly belonging to six species: Aspergillus fumigatus, Paecilomyces variotii, Mucor pusillus, Thermomyces lanuginosus, Humicola grisea , and Thermoascus aurantiacus . Penicillium and Aspergillus , respectively, 32.3 and 26.9% of total isolates represented the majority of mesophilic fungi isolated. When considering total strains (including thermotolerant strains) Aspergillus were the predominant strains isolated; follow‐up studies on mycotoxins therefore focused primarily on aflatoxins (AFs) and ochratoxin A (OTA) from the latter strains. All isolated Aspergillus flavus strains (9) and Aspergillus niger strains (36) were studied in order to evaluate their capacity to produce AFs and OTA, respectively, when grown on starch‐based culture media. Seven of the nine tested A. flavus strains isolated from olive and olive cake produced AF B1 at concentrations between 48 and 95 μg/kg of dry rice weight. As for the A. niger strains, 27 of the 36 strains produced OTA.