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Anti‐human immunodeficiency virus activity of 3,4,5‐tricaffeoylquinic acid in cultured cells of lettuce leaves
Author(s) -
Tamura Hirotoshi,
Akioka Takashi,
Ueno Koichi,
Chujyo Takeshi,
Okazaki Katsuichiro,
King Peter J.,
Robinson W. Edward
Publication year - 2006
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200500216
Subject(s) - integrase , chlorogenic acid , virus , cell culture , human immunodeficiency virus (hiv) , biology , chemistry , biochemistry , virology , food science , genetics
Abstract 3,4,5–Tricaffeoylquinic acid (TCQA) that is not found in intact plant of lettuce leaves was isolated from the cultured cells. The intact plant produced chicoric acid (dicaffeoyl tartaric acid: L ‐CCA) as well as chlorogenic acid (3‐caffeoylquinic acid: 3‐CQA) as the major metabolites. After subculturing of the cells for 40 days, the amount of 3,4,5‐TCQA reached to 0.14 mg/g fresh weight. The inhibitory effect of 3,4,5‐TCQA for human immunodeficiency virus (HIV) Type 1 integrase was assayed. Anti‐HIV activity using HIV and MT‐2 cells was 1.15 μM and IC 50 against HIV integrase was 0.063 μM whereas cell toxicity of this chemical was expressed as 5% death of all living cells to be 18.4 μM. The HIV inhibitory effect of 3,4,5‐TCQA was the highest in values among L ‐CCA, and other dicaffeoylquinic acids. This data will provide a new possibility for creating a new drug design for HIV.