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Studies on apple and blueberry fruit constituents: Do the polyphenols reach the colon after ingestion?
Author(s) -
Kahle Kathrin,
Kraus Michael,
Scheppach Wolfgang,
Ackermann Matthias,
Ridder Friederike,
Richling Elke
Publication year - 2006
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200500211
Subject(s) - polyphenol , ingestion , food science , chemistry , anthocyanin , bilberry , high performance liquid chromatography , proanthocyanidin , sugar , biochemistry , chromatography , antioxidant
The aim of our studies was to determine the amount of polyphenols reaching the colon after oral intake of apple juice and blueberries. After a polyphenol‐free diet healthy ileostomy volunteers consumed a polyphenol‐rich cloudy apple juice while others consumed anthocyanin‐rich blueberries. Ileostomy effluent was collected and polyphenols were identified using HPLC‐DAD as well as HPLC‐ESI‐MS/MS; quantification was performed with HPLC‐DAD. Most of the orally administered apple polyphenols were absorbed from or metabolized in the small intestine. Between 0 and 33% of the oral dose was recovered in the ileostomy bags with a maximum of excretion after 2 h. A higher amount of the blueberry anthocyanins under study (up to 85%, depending on the sugar moiety) were determined in the ileostomy bags and therefore would reach the colon under physiological circumstances. Such structure‐related availability has to be considered when polyphenols are used in model systems to study potential preventive effects in colorectal diseases.