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Amplification and diversity analysis of ketosynthase domains of putative polyketide synthase genes in Aspergillus ochraceus and Aspergillus carbonarius producers of ochratoxin A
Author(s) -
Atoui Ali,
Phong Dao Huy,
Mathieu Florence,
Lebrihi Ahmed
Publication year - 2006
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200500165
Subject(s) - aspergillus ochraceus , polyketide synthase , ochratoxin a , aspergillus , polyketide , biology , ochratoxins , phylogenetic tree , ochratoxin , gene , primer (cosmetics) , genetics , microbiology and biotechnology , mycotoxin , botany , chemistry , biosynthesis , organic chemistry
Abstract The diversity of polyketide synthase (PKS) genes in Aspergillus ochraceus NRRL 3174 and Aspergillus carbonarius 2Mu134 has been investigated using different primer pairs previously developed for the ketosynthase (KS) domain of fungal PKSs. Nine different KS domain sequences in A. ochraceus NRRL 3174 as well as five different KS domain sequences in A. carbonarius 2Mu134 have been identified. The identified KS fragments were distributed in five different clusters on the phylogenetic tree, indicating that they most probably represent PKSs responsible for different functions.