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Effect of nisin on the growth of Staphylococcus aureus determined by a microcalorimetric method
Author(s) -
Wang Xiaohong,
Liu Ying,
Xie Bijun,
Shi Xianming,
Zhou Junchu,
Zhang Honglin
Publication year - 2005
Publication title -
molecular nutrition and food research
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.495
H-Index - 131
eISSN - 1613-4133
pISSN - 1613-4125
DOI - 10.1002/mnfr.200400079
Subject(s) - nisin , staphylococcus aureus , chemistry , bacterial growth , minimum inhibitory concentration , microbiology and biotechnology , food science , bacteria , biochemistry , biology , antimicrobial , in vitro , organic chemistry , genetics
A novel microcalorimetric technique based on the bacterial heat output was applied to evaluate the biological effect of nisin on the growth of Staphylococcus aureus . The thermogenic curves of S. aureus in the presence of nisin were studied by an LKB‐2277 Thermal Activity Monitor. The thermokinetic parameters, such as the growth rate constant ( k ), the generation times ( G ), the inhibitory ratio ( I ), and the half inhibitory concentration ( IC 50 ), for the growth of S. aureus at different nisin concentrations were determined. The relationship between the growth rate constant ( k ) and the concentration of nisin ( c ) is nearly linear, which can be modeled by the formula k = 0.03794 – 4.005×10 –4 × c , with a correlation coefficient of –0.9971. Based on this model, we obtained the critical inhibitory concentration of nisin on the growth of S. aureus at 94.73 IU/mL. We proposed that this microcalorimetric method could be a useful tool in monitoring the biological effect of nisin on microorganisms, and providing valuable information on the study of microorganism metabolisms.