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Evaluation of dual release of stromal cell‐derived factor‐1 and basic fibroblast growth factor with nerve conduit for peripheral nerve regeneration: An experimental study in mice
Author(s) -
Shintani Kosuke,
Uemura Takuya,
Takamatsu Kiyohito,
Yokoi Takuya,
Onode Ema,
Okada Mitsuhiro,
Tabata Yasuhiko,
Nakamura Hiroaki
Publication year - 2020
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.30548
Subject(s) - basic fibroblast growth factor , nerve guidance conduit , medicine , regeneration (biology) , stromal cell , sciatic nerve , axon , angiogenesis , fibroblast growth factor , nerve growth factor , tissue engineering , surgery , anatomy , growth factor , pathology , biomedical engineering , microbiology and biotechnology , biology , receptor
Background The development of drug delivery systems has enabled the release of multiple bioactive molecules. The efficacy of nerve conduits coated with dual controlled release of stromal cell‐derived factor‐1 (SDF‐1) and basic fibroblast growth factor (bFGF) for peripheral nerve regeneration was investigated. Materials and methods Sixty‐two C57BL6 mice were used for peripheral nerve regeneration with a nerve conduit (inner diameter, 1 mm, and length, 7 mm) and an autograft. The mice were randomized into five groups based on the different repairs of nerve defects. In the group of repair with conduits alone ( n = 9), a 5‐mm sciatic nerve defect was repaired by the nerve conduit. In the group of repair with conduits coated with bFGF ( n = 10), SDF‐1 ( n = 10), and SDF‐1/bFGF ( n = 10), it was repaired by the nerve conduit with bFGF gelatin, SDF‐1 gelatin, and SDF‐1/bFGF gelatin, respectively. In the group of repair with autografts ( n = 10), it was repaired by the resected nerve itself. The functional recovery, nerve regeneration, angiogenesis, and TGF‐β1 gene expression were assessed. Results In the conduits coated with SDF‐1/bFGF group, the mean sciatic functional index value (−88.68 ± 10.64, p = .034) and the axon number (218.8 ± 111.1, p = .049) were significantly higher than the conduit alone group, followed by the autograft group; in addition, numerous CD34‐positive cells and micro vessels were observed. TGF‐β1 gene expression relative values in the conduits with SDF‐1/bFGF group at 3 days (7.99 ± 5.14, p = .049) significantly increased more than the conduits alone group. Conclusion Nerve conduits coated with dual controlled release of SDF‐1 and bFGF promoted peripheral nerve regeneration.