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Axonal supercharged interpositional jump‐graft with a hybrid artificial nerve conduit containing adipose‐derived stem cells in facial nerve paresis rat model
Author(s) -
Kamei Wataru,
Matsumine Hajime,
Osaki Hironobu,
Ueta Yoshifumi,
Tsunoda Satoshi,
Shimizu Mari,
Hashimoto Kazuki,
Niimi Yosuke,
Miyata Mariko,
Sakurai Hiroyuki
Publication year - 2018
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.30389
Subject(s) - medicine , hypoglossal nerve , nerve guidance conduit , facial nerve , epineurial repair , regeneration (biology) , surgery , anatomy , adipose tissue , sciatic nerve , paresis , compound muscle action potential , electrophysiology , tongue , pathology , biology , microbiology and biotechnology
Purpose Interpositional jump‐graft (IPJG) technique with the hypoglossal nerve for supercharging can be applied in a facial nerve paresis case. In IPJG, an autologous nerve is required, and the donor site morbidity is unavoidable. Biodegradable nerve conduits are made from polyglycolic acid (PGA) and used recently without donor site complications after providing autologous grafts. Hybrid artificial nerve conduits with adipose‐derived stem cells (ASCs) also attract attention as a nerve‐regeneration enhancing agent. This study examined the effect of hybrid artificial nerve conduit on IPJG. Materials and methods A total of 34 Lewis rats were used and divided into 4 groups by the bridge materials: autograft ( n = 8), PGA nerve conduit ( n = 8), hybrid PGA nerve conduit with ASCs ( n = 8), and the nontreated control groups ( n = 8). ASCs were collected from 2 rats and cultured. The animals were assessed physiologically and histopathologically at 13 weeks after surgery. Results In compound muscle action potential, the amplitude of hybrid PGA group (3,222 ± 1,779 μV) was significantly higher than that of PGA group (1,961 ± 445 μV, P < .05), and no significant difference between hybrid PGA and autograft group. All treated groups showed a myelinated nerve regeneration with double innervation in hypoglossal and facial nerve nuclei for vibrissal muscle. Conclusion This study showed the effectiveness of IPJG with a hybrid PGA conduit especially in physiological examination.