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Cell lineage in vascularized bone transplantation
Author(s) -
Willems Wouter F.,
Larsen Mikko,
Friedrich Patricia F.,
Bishop Allen T.
Publication year - 2014
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.22147
Subject(s) - allotransplantation , medicine , transplantation , cortical bone , immunosuppression , bone remodeling , andrology , pathology , surgery , urology
Background The biology behind vascularized bone allotransplantation remains largely unknown. We aim to study cell traffic between donor and recipient following bone auto‐, and allografting. Methods Vascularized femoral transplantation was performed with arteriovenous bundle implantation and short‐term immunosuppression. Twenty male Piebald Virol Glaxo (PVG; RT1 c ) rats received isotransplants from female PVG (RT1 c ) rats and 22 male PVG rats received allografts from female Dark Agouti rats (DA, RT1 a ), representing a major histocompatibility mismatch. Both groups were randomly analyzed at 4 or 18 weeks. Bone remodeling areas (inner and outer cortical samples) were labeled and laser capture microdissected. Analysis of sex‐mismatch genes by real‐time reverse transcription‐polymerase chain reaction provided the relative Expression Ratio (rER) of donor (female) to recipient (male) cells. Results The rER was 0.456 ± 0.266 at 4 weeks and 0.749 ± 0.387 at 18 weeks ( p = 0.09) in allotransplants. In isotransplants, the rER was 0.412 ± 0.239 and 0.467 ± 0.252 at 4 and 18 weeks, respectively ( p = 0.21). At 4 weeks, the rER at the outer cortical area of isotransplants was significantly lower in isotransplants as compared with allotransplants (0.247 ± 0.181 vs. 0.549 ± 0.184, p = 0.007). Cells in the inner and outer cortical bone remodeling areas in isotransplants were mainly donor derived (rER < 0.5) at 18 weeks, whereas allotransplants contained mainly recipient‐derived cells (rER > 0.5) at 18 weeks. Conclusions Applying novel methodology, we describe detailed cell traffic in vascularized bone transplants, elaborating our comprehension on bone transplantation. © 2013 Wiley Periodicals, Inc. Microsc. Res. Tech. 34:37–43, 2013.