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Improved energetic recovery of skeletal muscle in response to ischemia and reperfusion injury followed by in vivo 31 P‐magnetic resonance spectroscopy
Author(s) -
Lundberg Jonas,
Lindgård Ann,
Elander Anna,
Soussi Bassam
Publication year - 2002
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.21744
Subject(s) - phosphocreatine , medicine , ischemia , skeletal muscle , perfusion , in vivo , adenosine triphosphate , laser doppler velocimetry , reperfusion injury , anesthesia , magnetic resonance imaging , high energy phosphate , energy metabolism , blood flow , microbiology and biotechnology , biology , radiology
It is of great clinical interest to improve postischemic tissue recovery during microsurgical transfers. The effect of singlet oxygen energy (SOE) as photon illumination at 634 nm on rat skeletal muscle during ischemia and postischemic reperfusion was investigated noninvasively and continuously by in vivo 31 P‐magnetic resonance spectroscopy ( 31 P‐MRS). A model of pedicled rat rectus femoris muscle was used, where phosphorous metabolites were followed before onset of ischemia (control), after 4 h of ischemia, and after 1 h of reperfusion. Two groups were studied: one control group (n = 10), and one SOE‐treated group (n = 10). Blood perfusion was measured by laser Doppler flowmetry (LDF) during the study. After 4 h of ischemia, ATP levels were 72% and 51% of normal control values in the illuminated group and the control group, respectively ( P < 0.05). After 1 h of postischemic reperfusion, phosphocreatine (PCr) recovered to 79% and adenosine triphosphate (ATP) to 71% in the illuminated group, whereas in the control group, the recovery was 57% and 51%, respectively ( P < 0.05). It is concluded that singlet oxygen energy has a beneficial effect on the energy state of skeletal muscle during ischemia and postischemic reperfusion. © 2002 Wiley‐Liss, Inc. MICROSURGERY 22:158–164 2002