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Biliary fibrosis in microsurgical extrahepatic cholestasis in the rat
Author(s) -
SánchezPatán Fernando,
Anchuelo Raquel,
Corcuera MaríaTeresa,
Casado Isabel,
GómezAguado Fernando,
Aller MaríaAngeles,
Cruz Arturo,
Alonso MaríaJosé,
Arias Jaime
Publication year - 2008
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.20495
Subject(s) - medicine , cholestasis , gastroenterology , fibrosis , portal venous pressure , portal hypertension , collateral circulation , hepatic fibrosis , bile duct , bilirubin , biliary tract , pathology , cirrhosis
A new model of extrahepatic cholestasis, using a microsurgical technique, is performed as an alternative to the traditional model of the bile duct ligated‐rat, in order to study the stage of fibrosis in the long‐term. Male Wistar rats were divided into two groups: I (Sham‐operated, n = 9) and II [Microsurgical Cholestasis (MC), n = 10]. After 4 weeks, portal pressure, types of portosystemic collateral circulation, mesenteric venous vasculopathy, hepatic function test, and liver histopathology were studied by using the Knodell index and fibrosis was determined by reticulin and Sirius red stains. The animals with MC presented portal hypertension with extrahepatic portosistemic collateral circulation, associated with mesenteric venous vasculopathy and increased plasma levels of bilirubin (6.30 ± 1.80 vs. 0.22 ± 0.37 mg/dL; P = 0.0001), alkaline phosphatase (293.00 ± 82.40 vs. 126.30 ± 33.42 U/L; P = 0.001), AST (380.00 ± 78.50 vs. 68.33 ± 11.74 IU/L; P = 0.0001), ALT (87.60 ± 22.32 vs. 42.22 ± 7.89 IU/L; P = 0.0001), and LDH (697.76 ± 75.13 vs. 384.80 ± 100.03 IU/L; P = 0.0001). On the contrary, plasma levels of albumin decreased (2.72 ± 0.12 mg/dl vs. 2.99 ± 0.10; P = 0.001). The microsurgical resection of the extrahepatic biliary tract in the rat produces an experimental model of hepatic inflammation, characterized by a high Knodell hepatic activity index (4), bile proliferation, and fibrosis. © 2008 Wiley‐Liss, Inc. Microsurgery, 2008.