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Impact of cold preservation on leukocyte adhesion to the transplanted rat lung
Author(s) -
Enomoto Aki,
Kikuchi Takeshi,
Seo Norimasa,
Matsuno Kenjiro,
Kobayashi Eiji
Publication year - 2007
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.20341
Subject(s) - medicine , lung , in vivo , immunohistochemistry , microsurgery , transplantation , pathology , adhesion , lung transplantation , reperfusion injury , ischemia , surgery , biology , chemistry , microbiology and biotechnology , organic chemistry
Little is known about leukocyte migration in the early phase after ischemia‐reperfusion injury. Recently, there has been much focus on in vivo visualization of cell kinetics. Previously, we established a method for the in vivo visualization of leukocyte circulation using a Green Fluorescent Protein transgenic rat. Herein, we developed a simplified rat heterotopic lung transplantation model that can be mastered by beginners within a few weeks. Using this system, we studied the impact of preservation on adhesion of circulating leukocytes to the transplanted lung. The graft of a fresh group was compared with that of a preserved group. Morphological and immunohistochemical analyses were also performed. We successfully visualized few adherent leukocytes in the fresh graft, whereas adherent cells were attached in the preserved graft within a few minutes. In conclusion, the prolonged cold preservation time promotes leukocyte adhesion, resulting in increased microvascular injury after lung transplantation. © 2007 Wiley‐Liss, Inc. Microsurgery 2007.