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Regenerative capacity of individual liver lobesin the microsurgical mouse model
Author(s) -
Inderbitzin Daniel,
Studer Peter,
Sidler Daniel,
Beldi Guido,
Djonov Valentin,
Keogh Adrian,
Candinas Daniel
Publication year - 2006
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.20271
Subject(s) - liver regeneration , lobe , regeneration (biology) , bromodeoxyuridine , medicine , liver lobe , microsurgery , caudate lobe , hepatectomy , resection , pathology , anatomy , surgery , biology , immunohistochemistry , microbiology and biotechnology
In the development of microsurgical mouse models of hepatic regeneration and repair, lobe‐specific regenerative responses were observed. We therefore determined the hepatic regenerative capacity of individual mouse liver lobes. In mice, 26, 60, 75, and 83% of total liver mass was resected. Bromo‐deoxyuridine (BrdU) was injected prior to liver harvest and the BrdU labeling index determined in all remaining individual liver lobes. BrdU‐positive nuclei were seen in all liver lobes after the 26 and 60% resection, but significantly fewer were detected in the caudate lobe. In the 75% group, equally distributed positive nuclei were found. However, BrdU labeling was scant in the 83% group. In microsurgical mouse liver‐regeneration models, the average hepatic response depends on amount of liver tissue resected and on the remaining liver lobe. BrdU incorporation can vary significantly among individual lobes. The lobe‐specific differences observed may prove valuable in further investigations of hepatic regeneration and repair. © 2006 Wiley‐Liss, Inc. Microsurgery, 2006.