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Improved technique of mouse heterotopic heart graft retransplantation
Author(s) -
Wang Kunjie,
Zhang Nan,
Li Hong
Publication year - 2006
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.20219
Subject(s) - medicine , heart transplantation , surgery , cuff , transplantation , heart failure , anesthesia , cardiology
Research on chronic graft failure requires a graft retransplantation model to elucidate the affects of early transplant episodes on long‐term graft survival. Prolonged ischemic time is the major hurdle for this retransplant model. Here we describe a novel technique of mouse heart retransplantation, with a shortened retransplant ischemic time and higher success rate. The primary heart transplant employed an allogeneic abdominal heart‐transplant technique (Balb/C to C57BL/6 mice). Thirty to 60 days later, the graft was reharvested and retransplanted into the second syngeneic recipient (Balb/C mice) by using cervical heterotopic heart transplantation with a cuff technique. The total ischemic time of retransplant was reduced to within 35 min with the cuff technique, and a greater retransplant success rate (90.6%) was achieved. This heart retransplantation technique is a novel, useful tool in transplantation research. © 2006 Wiley‐Liss, Inc. Microsurgery, 2006.