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Murine fetal small‐intestine grafts: Morphometric and immunohistochemical evaluation
Author(s) -
Saldanha De Almeida Carlos Eduardo,
Ferreira De Oliveira Kátia,
Bochetti Manna Mônica Cecília,
Artigiani Ricardo,
Koike Márcia Kiyomi,
De Souza Montero Edna Frasson
Publication year - 2006
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.20213
Subject(s) - medicine , immunohistochemistry , fetus , small intestine , pathology , anatomy , pregnancy , biology , genetics
We investigated histopathological changes following murine fetal intestinal transplantation. Fetal intestine, obtained from a pregnant C57BL/6 mouse, was transplanted into BALB/c and C57Bl/6 mice. Recipients were divided into three groups: isogeneic, and allogeneic treated with 3 mg/kg/day gangliosides (Allo‐a) or 9 mg/kg/day (Allo‐b). One week after transplant, all grafts showed good viability, confirmed by cellular mitosis in the mucosa and a well‐defined propria muscular layer. Isogeneic grafts showed a thicker muscular layer than in the Allo‐a ( P = 0.02) and Allo‐b ( P = 0.004) groups. There was no difference in number of mitotic cells among groups. Goblet cells were significantly reduced in allografts treated with 3 mg gangliosides ( P = 0.013) or 9 mg gangliosides ( P = 0.002) compared to isografts. Villi height was similar in all studied groups. There was no difference in positivity of the enteric nervous system among groups. Atrophy was more common in the allogeneic groups, suggesting that isografts had better development than allografts treated with gangliosides. © 2006 Wiley‐Liss, Inc. Microsurgery 26: 61–64, 2006.