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Simple and viable in vitro perfusion model for training microvascular anastomoses
Author(s) -
Krishnan Kartik G.,
Dramm Peter,
Schackert Gabriele
Publication year - 2004
Publication title -
microsurgery
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.031
H-Index - 63
eISSN - 1098-2752
pISSN - 0738-1085
DOI - 10.1002/micr.20031
Subject(s) - microvessel , medicine , anastomosis , perfusion , in vitro , microsurgery , blood vessel , catheter , surgery , pathology , anatomy , cardiology , biology , immunohistochemistry , biochemistry
In this report, we describe a novel in vitro perfused microvessel model for training microvascular anastomotic exercises. Arteries and veins with a diameter of ca. 1 mm were explanted from chicken wings. These vessels were cannulated at both ends and mounted on a platform. Preserved, expired whole blood obtained from the blood bank was continuously injected through the proximal catheter, using an automatic perfusor. This in vitro perfused microvessel model exactly simulated the viable small‐animal vessels. The setting is very simply and reliably repeated; the materials used are very cheap and universally available. There are no ethical questions involved. Vessels explanted from the human placenta or omentum may be used in a similar manner to gain the “feel” of functioning human microvascular tissue. But such materials are rarer and require the approval of ethical committees. © 2004 Wiley‐Liss, Inc.